Rapid Fire Abstracts
Fabao Gao, MD
Radiology, Medical Imaging West China School of Medicine, Sichuan University, China (People's Republic)
Fabao Gao, MD
Radiology, Medical Imaging West China School of Medicine, Sichuan University, China (People's Republic)
Hongke Yin, MD
No. 37 Guoxue Road, Chengdu 610041, China
Department of Radiology, West China Hospital, Sichuan University, China (People's Republic)
Boshen Liang, MD
Department of Radiology, West China Hospital
West China Hospital, China (People's Republic)
haotian chen, MD, PhD
Department of Radiology, West China Hospital
West China Hospital, China (People's Republic)
xin fang, MD, PhD
Department of Radiology, West China Hospital
West China Hospital, China (People's Republic)
bojun chen, PhD
Yunnan University of Chinese Medicine
Yunnan University of Chinese Medicine, China (People's Republic)
lei wang, MD, PhD
Department of Radiology, West China Hospital
Department of Radiology, West China Hospital, China (People's Republic)
Cang-ai volatile oil (CAVO) is a traditional Chinese medicine (TCM) by combining Chinese medicine theory and modern pharmaceutical technology. Despite the CAVO can reduce damage of cardiac in rats in plains, its effect on animals at high altitude has seldom been reported. We hypothesize that cardiac damage model induced by chronic exposure to hypobaric hypoxia and myocardial hypertrophy (MH) induced by Isoproterenol (ISO) at high altitude will be ameliorated by using CAVO.
Methods: Seventy rats were randomized into the Control group (CON), hypobaric hypoxia (HH group), HH+ CAVOh (hypobaric hypoxia + CAVOh), HH+ CAVOl(hypobaric hypoxia + CAVOl), HH+MH (hypobaric hypoxia+ myocardial hypertrophy,HM), HM+CAVOh, HM+CAVOl. Except the Control group (altitude: 500 m), rats in other groups were transported to Yushu (altitude: 4,250m) for two months, where the group of HM,HM+CAVOh,HM+CAVOl were underwent intraperitoneal injection of ISO (3 mg/kg for 14 days) and HH, HH+CAVOh, HH+CAVOl group were underwent intraperitoneal injection of saline in the same time. Left ventricular function, global strain and the degree of myocardial edema of the rats were measured by 7.0T high-field CMR and analyzed using the cine tissue tracking techniques. Biochemical tests, histopathology and electronic microscopy were used to evaluate the protective effect of CAVO on the heart tissue of cardiac damage rats exposed to a high-altitude environment.
Results:
The left ventricular ejection fraction (LVEF) and global strains were improved in all group after treat by CAVO compared with the Hypobaric Hypoxia group (p＜0.05). Furthermore, the oxidative stress injuries were after CAVO treatment, evidenced by the increases of SOD, GSH-Px, while the decreases of MDA and LDH contents (all p< 0.05).Concurrently, the results of western blot indicated that CAVO treatment dramatically restrained Keap, COL-1, α-SMA protein expressions in cardiac tissues of mice，at the same time, NRF2,HO-1 protein expression were increased.
Conclusion:
CAVO can improve cardiac damage caused by hypobaric hypoxia and ventricular hypertrophy induced by ISO at high altitude through oxidative stress-related indicators, effect can be detected by 7.0T high-field CMR.
